This study was conducted to determine the effect of organizational culture on employee happiness. A self-reported survey examined organizational culture and the levels of employee happiness at work. For this study, 59 employed top leaders, management, and workers of faith-based higher education institutions were surveyed to measure the effect of organizational culture on employee happiness. Factor analysis was performed to create a variable to evaluate organizational culture (the reliability for organizational culture was 92.8%). The happiness at work variable was divided into two groups (happy and unhappy) based on a specific response. An independent sample t-test and descriptive statistics were conducted. The data were split to compare groups and descriptive frequencies were analyzed. Organizational culture was found to be statistically significant on employee happiness. An item-by-item analysis was performed. The results indicate that unhappy employees have the highest disagreement in statements such as "coworkers being nonjudgmental" (66.7%), "leaders are aware of the needs of others" (38%), and "leaders are humble and do not promote themselves" (50%). However, happy employees unanimously responded to most items. Therefore, happy people were more likely to be more consistent. The mean for happy people was 76.48 with a standard deviation of 9.56. The mean for unhappy people at work was 58.22 with a standard deviation of 15.33. This study was highly statistically significant with a p value of 0.00.