Unlike the transitions children make between settings, those they undertake between age groups within early childhood care and education (ECCE) settings are seldom studied. Accordingly, this exploratory study followed seven preschool children (three boys and four girls) as they moved to new rooms in five ECCE settings. Structured observations of children's behaviour were collected along with semi-structured parent interviews and participatory child interviews. All boys and one girl demonstrated increased anxiety behaviours following transitions. This gender difference was mirrored in parental reports of negative affect and aggression in sons, but independence and assertiveness amongst daughters. Families also reported shifts in children's identity from expert to novice and a sense of becoming "big." Interviews highlighted the challenges and opportunities underlying transitions, and parents provided a rich overview of the factors they believed support and hinder transitions, emphasising the importance of strong home-centre connections. These exploratory findings suggest that internal ECCE transitions may be unique junctures in children's ECCE experiences.