This research investigated the level of knowledge and perceptions of trainee teachers towards the LGBTQ+ community. In order to achieve this, a descriptive study was carried out, with the objective of becoming aware of the expectations and preconceptions held by trainee teachers in the fourth year of their degree course in Primary and Infant Education during the 2018-2019 session. In addition, limitations of the educational approach and the keys to awareness and acceptance in school, of sexual and gender diversity, were also investigated. In order to collect the data a questionnaire instrument was used and a discussion group was also identified. The key results revealed that trainee teachers do understand the reality of this community and are disposed in favour of the acceptance of sexual and gender diversity in the classroom. In fact, they demonstrated the need for important training in order to provide an effective approach to sexually related material.