This study aimed to examine the effect of interactive videos on volleyball education, and the effects of gender and grade level on volleyball knowledge level were evaluated. A total of 105 (43 boys and 62 girls) fifth and sixth-grade students participated in the study. Participants were divided into three groups: Interactive Video Group (IVG), Video Group (VG), and Control Group (CG). The volleyball education program consisting of seven videos for two weeks was applied to the experimental groups. Interactive videos include single or multiple-choice questions, true-false statements, and drag and drop activities. ADDIE Model was used in instructional design. Gender has no effect on volleyball knowledge level (p>0.05), but grade level has an effect in favor of sixth graders (p<05). In terms of volleyball knowledge level, there is a difference between the pretest and posttest of IVG and VG (p<0.05) but not in CG (p>0.05). There is a difference in favor of IVG and VG, respectively, in posttest results (p<0.05), but not in pretest results (p>0.05). Interactive videos positively affect volleyball knowledge, so their use in educational environments should be encouraged.