The purpose of this study was to analyze the digital competence of teachers on the use of YouTube as a didactic resource in the teaching and learning process of students. Specifically, this was analyzed and compared for each educational stage (Preschool Education, Primary Education, Secondary Education, Adult Education, and Vocational Education-VET) between genders of the teacher (female and male). For this, an ex post facto non-experimental design was used, with a sample of 2157 in-service teachers from all over Spain. Among the main results found in this study, teachers of all educational stages had satisfactory skills on the use of YouTube to search and communicate information, although low in relation to the creation of audio-visual material through YouTube. In addition, it was evidenced how gender was a significant factor which affected the competencies of teachers, in most of the educational stages analyzed.