Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and…

Two misconceptions about the mechanism of image formation in the human eye are common among students and even in textbooks and other teaching materials. The first attributes all refraction to the eye lens; the second treats the eye as a pinhole camera. To reduce these persistent conceptions of students, a series of simple experiments is presented…

Titration is an important experimental skill that every chemistry educator and learner must master. Titration is often the method of choice in the science laboratory courses in secondary and postsecondary schools. During acid-base titration, chemistry learners are always told about salt formation. However, a practical visualization of the salt…

A Fourier transform infrared (FTIR) experiment appropriate for an upper-level undergraduate laboratory such as chemical instrumentation is described. Students collect FTIR spectra of four protio-solvents and their deuterated analogues. In addition to qualitatively observing C-H and O-H peaks shift to lower energy upon deuteration, students apply a…

The CRISPR/Cas9 system is widely used for editing genes in various organisms and is a very useful tool due to its versatility, simplicity, and efficiency. To teach its principles to post-graduate students we designed a laboratory activity to obtain and analyze PDS3 mutants in "Arabidopsis thaliana" plants consisting of: 1) Design of…

Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how microbes switch the flux of carbon towards alternative metabolic fates as a function of nutrient availability and according to…

Chemistry is considered a difficult subject by most students. Its difficulty lies in Chemistry's complex and abstract nature. This highly abstract nature requires constant interplay and coordination between the macroscopic, particulate, and symbolic representations. Experts can successfully navigate the various representations without overloading…

Laboratory tasks often focus on mechanical procedures leaving limited time and opportunities for students to build conceptual knowledge. We investigate to what extent introducing simulation tasks to preparation work can enable students to build their conceptual knowledge. We surveyed two cohorts of students taking an electronics module. Laboratory…