Cross-cultural equivalence, country and gender differences, and external relations with other measures were examined on a new, brief measure of test anxiety, the Test Anxiety Measure for College Students-Short Form (TAMC-SF), in a sample of Canadian and US higher education students. The sample of 1204 students completed the TAMC-SF and other measures online. The results of tests of invariance found support for partial scalar invariance across country and gender on the TAMC-SF. In addition, results of a multivariate analysis of variance (MANOVA) and analysis of variances (ANOVAs) found country and gender differences on the TAMC-SF scales. Furthermore, validity evidence for the TAMC-SF scores with the scores of external measures was found. Overall, the findings support the use of the same test score interpretation for Canadian and US higher education students on the TAMC-SF and the use of the TAMC-SF in Canadian higher education students.