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What Is the True Color of Fresh Meat? A Biophysical Undergraduate Laboratory Experiment Investigating the Effects of Ligand Binding on Myoglobin Using Optical, EPR, and NMR Spectroscopy
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Linenberger, Kimberly; Bretz, Stacey Lowery; Crowder, Michael W.; McCarrick, Robert; Lorigan, Gary A.; Tierney, David L. – Journal of Chemical Education, 2011
With an increased focus on integrated upper-level laboratories, we present an experiment integrating concepts from inorganic, biological, and physical chemistry content areas. Students investigate the effects of ligand strength on the spectroscopic properties of the heme center in myoglobin using UV-vis, [superscript 1]H NMR, and EPR…
Descriptors: Inorganic Chemistry, Biology, Physics, Chemistry
A Guided Inquiry Experiment for the Measurement of Activation Energies in the Biophysical Chemistry Laboratory: Decarboxylation of Pyrrole-2-Carboxylate
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Hutchinson, Kelly M.; Bretz, Stacey Lowery; Mettee, Howard D.; Smiley, Jeffrey A. – Biochemistry and Molecular Biology Education, 2005
A laboratory experiment for undergraduate biophysical chemistry is described, in which the acid concentration and temperature dependences of the decarboxylation of pyrrole-2-carboxylate are measured using a continuous ultraviolet (UV) spectrophotometric assay. Data collection and analysis are structured using principles of guided inquiry. Data…
Descriptors: Laboratory Experiments, Chemistry, College Science, Undergraduate Students