Discusses a simulation on enzyme kinetics based upon the ability of computers to generate random numbers. The program includes: (1) enzyme catalysis in a restricted two-dimensional grid; (2) visual representation of catalysis; and (3) storage and manipulation of data. Suggested applications and conclusions are also discussed. (DH)

Describes an undergraduate enzyme kinetics experiment which uses bovine erythrocyte carbonic anhydrase, a very stable enzyme commercially available in lyophilized form. Includes background information, reactions involved, procedures used, and the calculation of typical results obtained. (JN)

Background information, procedures used, typical results obtained, and calculations are provided for a short (4-6 hours) kinetic experiment which includes different methods both in performance and in analysis of the results. The experiment can be modified and extended depending on the level of students and the available laboratory time. (JN)

The Briggs-Haldane assumption is used as the basis for the development of a kinetic model for enzyme catalysis. An alternative definition of the steady state and examples of realistic mechanisms are provided. (KR)

Describes an open-ended experiment involving a bioinorganic system that provides the student a direct link between thermodynamics and kinetics. It utilizes a rapid reaction technique and requires one to clearly understand the relationship of experimental observable (absorbance) to a mechanism. (GS)

Conveys an appreciation of enzyme kinetic analysis by using a practical and intuitive approach. Discusses enzyme assays, kinetic models and rate laws, the kinetic constants (V, velocity, and Km, Michaels constant), evaluation of V and Km from experimental data, and enzyme inhibition. (CW)

The confusion by students on the use of three terms in their writing is discussed. The definitions of catabolite repression, constitutive, and ribosome binding site, given in the glossaries of 10 textbooks, are compared with the use of these terms in students' papers. (KR)

Describes an undergraduate biological chemistry laboratory experiment which provides students with an example of pseudo-first-order kinetics with the cardiac glycoside inhibition of mammalism sodium and potassium transport. (SL)

Describes a set of experiments (for senior-level biochemistry students) which permit evaluation and estimation of rate and equilibrium constants involving an intermediate in the alpha-chymotrypsin mediated hydrolysis of ortho-hydroxy-alpha-toluenesulfonic acid (I). The only equipment required for the experiments is a well-thermostated double beam…

Describes an experiment to examine the kinetics of carbamate decomposition and the effect of buffer catalysis on the reaction. Includes background information, laboratory procedures, evaluation of data, and teaching suggestions. (Author/JN)

Examines the influence of enzyme and substrate concentrations, pH, temperature, and inhibitors on catalytic activity. Follows the influence of different phosphate concentrations in the growth medium on enzyme activity. Studies regulation of enzyme synthesis by repression. Includes methodology for six experiments. (MVL)

Describes a program which allows students to identify and characterize several kinetic inhibitory mechanisms. Uses the generic model of reversible inhibition of a monosubstrate enzyme but can be easily modified to run other models such as bisubstrate enzymes. Uses MS-DOS BASIC. (MVL)

Describes a biophysical chemistry experiment that introduces students to globular protein conformation and microcomputer analysis of initial rate data for the unfolding of proteins. Presents background, materials needed and methodology. Uses a visible spectrometer for analysis. Lists educational benefits derived from the experiment. (ML)