This study examined the relation between interparental conflict and cortisol recovery, with child temperamental negative affectivity as the moderator. Children (n = 118) ages 9-11 years observed an argument between their parents in the lab and provided saliva samples for cortisol assays. Children also reported levels of interparental conflict, and mothers reported their child's negative affectivity. Results showed that youths with high levels of negative affectivity experienced less cortisol recovery as interparental conflict levels increased. Further, demonstrating some support for differential susceptibility, youths with higher levels of negative affectivity exhibited less recovery at high levels of parental conflict but greater recovery at low levels of parental conflict, compared to peers with lower scores on negative affectivity. We also found that children with low levels of negative affectivity exhibited greater recovery as rates of interparental conflict increased. In summary, this study sheds light on sources of variability in cortisol recovery as it relates to interparental disharmony.