The purpose of the study was to assess the impact of staff training and development on employee job satisfaction among administrative staff in selected private universities in Uganda. This study was undertaken due to the increasing concerns of job discontentment among staff in private universities, especially administrative staff. The study was conducted in 5 selected private universities with main campuses in either Northern or Eastern Uganda. Useful data were solicited from 215 respondents who were non-teaching staff in the category of administrators from a population of 495. The unit of analysis was 5 selected private universities with main campuses in Eastern and Northern Uganda, while the unit of inquiry was administrative staff (non-academic staff). A descriptive design with a cross-sectional survey was used. This involved using a self-administered survey protocol for obtaining primary data. Respondents were obtained using simple random and purposive sampling techniques. Primary data were obtained using closed-ended structured questionnaires, and this generated a 100% response rate. The Statistical Package for Social Scientists (SPSS) 27 and Stata statistics software 14.2 were used for data analysis. The results confirm that staff training and development significantly predict employee job satisfaction ([beta] = 0.688**; ? < 0.01). Private universities need to prioritize training and development for non-teaching staff. Universities need to create a favorable atmosphere that encourages on-the-job training and further studies.