Explains the procedures used to determine the regulatory properties of yeast pyruvate kinase. Involves a partial purification using PEG precipitation that can be done in one laboratory period with simple equipment. (DDR)

Background information, experimental approach, materials needed, procedures used, and typical results obtained are provided for genetic engineering experiments. The course in which these experiments are performed is also described. (JN)

Describes an experiment which allows students to identify the nature of the time-course for chymotrypsin and to obtain mechanistic information on the two kinetic phases in terms of their concentration dependence. Also describes the computer simulation (available from the author) used in the experiment. (JN)

Background information, procedures used, typical results obtained, and calculations are provided for a short (4-6 hours) kinetic experiment which includes different methods both in performance and in analysis of the results. The experiment can be modified and extended depending on the level of students and the available laboratory time. (JN)

Presents an experiment aimed at constructing a genomic library that can be carried out over a week. Helps students learn concepts such as donor and vector DNAs, construction of recombinant DNA, host strain, and experiments in gene cloning more clearly. (PVD)

Demonstrates how leaf slices provide an inexpensive material for illustrating several fundamental points about the biochemistry of photosynthesis and respiration. Presents experiments that illustrate the effects of photon flux density and herbicides and carbon dioxide concentration. (DDR)

Describes an instructional experiment that was designed to illustrate the use of periodate oxidation in sugar analysis, and the consequence that the absorbance of mixtures of non-interacting substances is additive. (TW)

Describes a science activity designed for undergraduate biochemistry classes. Provides background, a list of materials, experimental procedures, and the results of this experiment. (CW)

Presents a practical exercise that enables students to measure flux control coefficients in a metabolic pathway in vitro, to verify the summation property, and to compare the differences in the distribution of these coefficients in different steady states. Discusses background, materials, methods, and results of the activity. (CW)

Suggests a new experiment to study the genetic material of eukaryotes. It is based on the rapid growth and interest of the research on chromatin. (TW)

Describes an experiment to identify lactose and estimate the concentration of lactose in a sample of milk. Gives a background of the investigation. Details the experimental method, results and calculations. Discusses the implications of the experiment to students. Suggests further experiments using the same technique used in…

Describes a three-week sequence to take undergraduate students through the study of enzyme kinetics in an integrated manner that reinforces the basic concepts of initial velocity and the effects of varying operational parameters. Discusses laboratory sessions and the use of a microcomputer in instruction. (CW)

Describes two complementary laboratory exercises that use the glass fiber assay to assess receptor specificity and hormone binding affinity in rat liver cytoplasmic extracts. Details the methods, materials and protocol of the experiments. Discusses the basic concepts illustrated and the feasibility of using the experiments at the undergraduate…

Describes an experiment to discover the topology of plasma membrane aminophospholipids (phosphatidylethanolamine and phosphatidylserine) using whole platelets and trinitrobenzene sulfonate (TNBS) as a probe. Reports changes in phospholipid distribution during platelet activation with simultaneous action of thrombia and collagen. Details the…

Presents a hand-out given to students at the University of Canterbury, New Zealand, concerning expectations and guidelines for finishing laboratory reports. Topics covered are accuracy, data collection, and write-up format that includes introduction, materials utilized, procedures, results, discussion, conclusion, tables and illustrations, units…